6-Cys Domains are also known as PGSH Domains1 and annotated as "s48_45" in SMART and Pfam

A NEW SET OF MODELS IS IN PROGRESS, in reflection of the very latest structural knowledge (Ref.2 and 3. below)
(mid-to-end of May 2017)

Background: We build, and update, the "next" generation of double 6-Cys domain models available here based on several recently solved
Plasmodium 6-Cys domain structures2-4. In 2012, the first experimentally determined structure4 had confirmed our tertiary structure prediction
for the 6-Cys Domain, and fold resemblance to the SRS protein family ("SAG"), six years after we published in 20055. A carefully assembled
and adjusted multiple sequence alignment of all 6-Cys domains is continually updated to reflect current-day knowledge, and enables us to
provide best-currently-achievable quality models for all family members and species. Click on the tabs above to access models for each species.

Please do not hesitate to contact us if you have any technical questions, or if you would like some help with how 3-D structural information may help your research.
D Gerloff, E Draizen

  1. Dietlind L Gerloff, Edward Liaw, Eli Draizen, David S Hall, Jonathan Magasin, Felicia Kemp, Richard Carter (2012)
    The evolutionary link between Coccidian SRS proteins and the malarial "6-Cys Domain" family: new clues from modeled 3-D structures [Abstract 2012]

  2. Parker ML, Peng F, Boulanger MJ (2015)
    The structure of Plasmodium falciparum blood-stage 6-Cys protein Pf41 reveals an unexpected intra-domain insertion required for Pf12 coordination.
    PLoS ONE Sep 28;10(9):e0139407. [Full Text]

  3. Favuzza P, et al (2017)
    Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invation inhibitory antibody.
    eLife Feb 14;10.7554/eLife 21347. [Abstract, accepted manuscript]

  4. Annoura T, van Schaijk BC, Ploemen IH, et al (2014)
    Two Plasmodium 6-Cys family-related proteins have distinct and critical roles in liver-stage development.
    FASEB J May;28(5):2158-70. [Full Text]

  5. Tonkin ML, Arredondo SA, Loveless BC, Serpa JJ, Makepeace KA, Sundar N, et al. (2013)
    Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement ith Pf41.
    J Biol Chem May 3;288(18):12805-12817. [Full Text].

  6. Arredondo SA, Cai M, Takayama Y, MacDonald NJ, Anderson DE, Aravind L, Clore GM, Miller LH (2012)
    Structure of the Plasmodium 6-cysteine s48/45 domain.
    PNAS Apr 24;109(17):6692-7. [Full Text]

  7. Dietlind L. Gerloff, Alison Creasey, Siarhei Maslau, Richard Carter (2005)
    Structural models for the protein family characterized by gamete surface protein Pfs230 of Plasmodium falciparum
    PNAS Sep;102(38):13598-603. [Full Text]

  8. Gilson PR, Nebl T, Vukcevic D, Moritz RL, Sargeant T, Speed TP, Schofield L, Crabb BS (2006)
    Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum.
    Mol Cell Proteomics Jul;5(7):1286-99. [Full Text]

Loading Plasmodium falciparum 3D7...
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Loading Plasmodium knowlesi strain H...
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Loading Plasmodium chabaudi chabaudi...
Loading Plasmodium yoelii yoelii 17XNL...

Foundation for Applied Molecular Evolution (FfAME) / UC Santa Cruz Biomolecular Engineering / University of Edinburgh
Also check out our Group's Malaria Genome Exploration Tool: MaGnET at

webstat For questions, comments or suggestions, please email Dietlind Gerloff:
or Eli Draizen: